AffiNGS

CAT Name AFG-PNG-01 High Throughput DNA Isolation AFG-PNG-02 5-Mc RNA Bisulfite-Sequencing (RNA BS-seq) for Illumina AFG-PNG-03 Bisulfite-Sequencing Kit for Illumina AFG-PNG-04 ChIP-Seq High-Sensitivity Kit for Illumina  AFG-PNG-05 CUT&RUN Fast Assay Kit AFG-PNG-06 CUT&RUN RNA m6A-Seq Kit  AFG-PNG-07 CUT&Tag In-Place (cTIP) Sequencing Kit AFG-PNG-08 DNA Library Preparation Kit for Illumina AFG-PNG-09 DNA Size Selection Kit AFG-PNG-10 … Read more

SARS-CoV-2 vaccination elicits unconventional IgM specific responses in naïve and previously COVID-19-infected individuals

 Currently, evaluation of the IgG antibodies specific for the SARS-CoV-2 Spike protein following vaccination is used worldwide to estimate vaccine response. Limited data are available on vaccine-elicited IgM antibodies and their potential implication in immunity to SARS-CoV-2.  We Bio Med Frontiers performed a longitudinal study to quantify anti-S SARS-CoV-2 IgG and IgM (IgG-S and IgM-S) in health care worker … Read more

Application of a Sanger-Based External Quality Assurance Strategy for the Transition of HIV-1 Drug Resistance Assays to Next Generation Sequencing

Application of a Sanger-Based External Quality Assurance Strategy for the Transition of HIV-1 Drug Resistance Assays to Next Generation Sequencing

The Nationwide Institute of Allergy and Infectious Ailments (NIAID) Virology High quality Assurance (VQA) established a sturdy proficiency testing program for Sanger sequencing (SS)-based HIV-1 drug resistance (HIVDR) testing in 2001. Whereas most of the classes discovered through the growth of such applications may additionally apply to subsequent technology sequencing (NGS)-based HIVDR assays, challenges stay … Read more

Next Generation Sequencing Reveals a Synchronous Trilateral Lung Adenocarcinoma Case with Distinct Driver Alterations of EGFR 19 Deletion or EGFR 20 Insertion or EZR-ROS1 Fusion

Next Generation Sequencing Reveals a Synchronous Trilateral Lung Adenocarcinoma Case with Distinct Driver Alterations of EGFR 19 Deletion or EGFR 20 Insertion or EZR-ROS1 Fusion

Goal: Synchronous a number of main lung most cancers (SMPLC) has a reported incidence from 0.5% to 2% in lung most cancers, and the surgical remedy and prognosis had been fairly numerous. With the invention of driver mutations in lung adenocarcinoma (ADC), next-generation sequencing (NGS) would offer an specific reply to the important thing query, whether … Read more

A highly sensitive method for the detection of recombinant PERV-A/C env RNA using next generation sequencing technologies

A highly sensitive method for the detection of recombinant PERV-A/C env RNA using next generation sequencing technologies

A number of xenogenic cell-based therapeutic merchandise are at present beneath improvement around the globe for the therapy of human illnesses. Porcine islet cell merchandise for treating human diabetes are a typical instance. Since porcine cells possess endogenous retrovirus (PERV), which may replicate in human cells in vitro, the potential transmission of PERV has raised … Read more

Next-generation Sequencing of Cerebrospinal Fluid for the Diagnosis of Unexplained Central Nervous System Infections

Next-generation Sequencing of Cerebrospinal Fluid for the Diagnosis of Unexplained Central Nervous System Infections

Background: Central nervous system infections trigger important morbidity and mortality in pediatric sufferers. Nevertheless, in roughly half of the scientific circumstances, the etiology is unidentified. As an unbiased molecular diagnostic know-how, next-generation sequencing is steadily being utilized to analyze central nervous system infections. This evaluate summarizes and critiques the literature on this new know-how for etiologic … Read more