AffiNGS

CAT Name AFG-PNG-01 High Throughput DNA Isolation AFG-PNG-02 5-Mc RNA Bisulfite-Sequencing (RNA BS-seq) for Illumina AFG-PNG-03 Bisulfite-Sequencing Kit for Illumina AFG-PNG-04 ChIP-Seq High-Sensitivity Kit for Illumina  AFG-PNG-05 CUT&RUN Fast Assay Kit AFG-PNG-06 CUT&RUN RNA m6A-Seq Kit  AFG-PNG-07 CUT&Tag In-Place (cTIP) Sequencing Kit AFG-PNG-08 DNA Library Preparation Kit for Illumina AFG-PNG-09 DNA Size Selection Kit AFG-PNG-10 … Read more

Application of a Sanger-Based External Quality Assurance Strategy for the Transition of HIV-1 Drug Resistance Assays to Next Generation Sequencing

Application of a Sanger-Based External Quality Assurance Strategy for the Transition of HIV-1 Drug Resistance Assays to Next Generation Sequencing

The Nationwide Institute of Allergy and Infectious Ailments (NIAID) Virology High quality Assurance (VQA) established a sturdy proficiency testing program for Sanger sequencing (SS)-based HIV-1 drug resistance (HIVDR) testing in 2001. Whereas most of the classes discovered through the growth of such applications may additionally apply to subsequent technology sequencing (NGS)-based HIVDR assays, challenges stay … Read more

Next Generation Sequencing Reveals a Synchronous Trilateral Lung Adenocarcinoma Case with Distinct Driver Alterations of EGFR 19 Deletion or EGFR 20 Insertion or EZR-ROS1 Fusion

Next Generation Sequencing Reveals a Synchronous Trilateral Lung Adenocarcinoma Case with Distinct Driver Alterations of EGFR 19 Deletion or EGFR 20 Insertion or EZR-ROS1 Fusion

Goal: Synchronous a number of main lung most cancers (SMPLC) has a reported incidence from 0.5% to 2% in lung most cancers, and the surgical remedy and prognosis had been fairly numerous. With the invention of driver mutations in lung adenocarcinoma (ADC), next-generation sequencing (NGS) would offer an specific reply to the important thing query, whether … Read more

A highly sensitive method for the detection of recombinant PERV-A/C env RNA using next generation sequencing technologies

A highly sensitive method for the detection of recombinant PERV-A/C env RNA using next generation sequencing technologies

A number of xenogenic cell-based therapeutic merchandise are at present beneath improvement around the globe for the therapy of human illnesses. Porcine islet cell merchandise for treating human diabetes are a typical instance. Since porcine cells possess endogenous retrovirus (PERV), which may replicate in human cells in vitro, the potential transmission of PERV has raised … Read more

Assessment of Minimal Residual Disease by Next Generation Sequencing in Peripheral Blood as a Complementary Tool for Personalized Transplant Monitoring in Myeloid Neoplasms

Assessment of Minimal Residual Disease by Next Generation Sequencing in Peripheral Blood as a Complementary Tool for Personalized Transplant Monitoring in Myeloid Neoplasms

Sufferers with myeloid neoplasms who relapsed after allogenic hematopoietic stem cell transplant (HSCT) have poor prognosis. Monitoring of chimerism and particular molecular markers as a surrogate measure of relapse isn’t all the time useful; subsequently, improved techniques to detect early relapse are wanted. We hypothesized that the usage of subsequent technology sequencing (NGS) may very … Read more

Next-generation Sequencing of Cerebrospinal Fluid for the Diagnosis of Unexplained Central Nervous System Infections

Next-generation Sequencing of Cerebrospinal Fluid for the Diagnosis of Unexplained Central Nervous System Infections

Background: Central nervous system infections trigger important morbidity and mortality in pediatric sufferers. Nevertheless, in roughly half of the scientific circumstances, the etiology is unidentified. As an unbiased molecular diagnostic know-how, next-generation sequencing is steadily being utilized to analyze central nervous system infections. This evaluate summarizes and critiques the literature on this new know-how for etiologic … Read more