Application of a Sanger-Based External Quality Assurance Strategy for the Transition of HIV-1 Drug Resistance Assays to Next Generation Sequencing

Application of a Sanger-Based External Quality Assurance Strategy for the Transition of HIV-1 Drug Resistance Assays to Next Generation Sequencing

The Nationwide Institute of Allergy and Infectious Ailments (NIAID) Virology High quality Assurance (VQA) established a sturdy proficiency testing program for Sanger sequencing (SS)-based HIV-1 drug resistance (HIVDR) testing in 2001. Whereas most of the classes discovered through the growth of such applications may additionally apply to subsequent technology sequencing (NGS)-based HIVDR assays, challenges stay … Read more

A highly sensitive method for the detection of recombinant PERV-A/C env RNA using next generation sequencing technologies

A highly sensitive method for the detection of recombinant PERV-A/C env RNA using next generation sequencing technologies

A number of xenogenic cell-based therapeutic merchandise are at present beneath improvement around the globe for the therapy of human illnesses. Porcine islet cell merchandise for treating human diabetes are a typical instance. Since porcine cells possess endogenous retrovirus (PERV), which may replicate in human cells in vitro, the potential transmission of PERV has raised … Read more

Assessment of Minimal Residual Disease by Next Generation Sequencing in Peripheral Blood as a Complementary Tool for Personalized Transplant Monitoring in Myeloid Neoplasms

Assessment of Minimal Residual Disease by Next Generation Sequencing in Peripheral Blood as a Complementary Tool for Personalized Transplant Monitoring in Myeloid Neoplasms

Sufferers with myeloid neoplasms who relapsed after allogenic hematopoietic stem cell transplant (HSCT) have poor prognosis. Monitoring of chimerism and particular molecular markers as a surrogate measure of relapse isn’t all the time useful; subsequently, improved techniques to detect early relapse are wanted. We hypothesized that the usage of subsequent technology sequencing (NGS) may very … Read more

Next-generation Sequencing of Cerebrospinal Fluid for the Diagnosis of Unexplained Central Nervous System Infections

Next-generation Sequencing of Cerebrospinal Fluid for the Diagnosis of Unexplained Central Nervous System Infections

Background: Central nervous system infections trigger important morbidity and mortality in pediatric sufferers. Nevertheless, in roughly half of the scientific circumstances, the etiology is unidentified. As an unbiased molecular diagnostic know-how, next-generation sequencing is steadily being utilized to analyze central nervous system infections. This evaluate summarizes and critiques the literature on this new know-how for etiologic … Read more