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VPK-219-ECO |
Cell Biolabs |
1 kit |
EUR 1163 |
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures. |
Cell Biolabs Laboratories manufactures the cell biolabs vpk-219-eco reagents distributed by Genprice. The Cell Biolabs Vpk-219-Eco reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact Cell Biolabs. Other Cell products are available in stock. Specificity: Cell Category: Biolabs Group: Vpk-219-Eco
ViraSafe Lentiviral Bicistronic Expression System (Puro), Ecotropic |
Cell Biolabs |
1 kit |
EUR 1163 |
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures. |
ViraSafe Lentiviral Bicistronic Expression System (Neo), Ecotropic |
Cell Biolabs |
1 kit |
EUR 1163 |
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures. |
ViraSafe Lentiviral Bicistronic Expression System (Hygro), Ecotropic |
Cell Biolabs |
1 kit |
EUR 1163 |
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures. |
pCMV-Eco Envelope Vector |
Cell Biolabs |
100 µL |
EUR 717 |
Description: Use this vector along with a packaging cell line and a cloning / expression vector for retroviral production. |
Vpk-219-Eco information
XMIR-219 RNA oligo miRNA-219a-5p with Xmotif |
XMIR-219 |
SBI |
10 rxn |
EUR 438 |
|
AXMIR-219 RNA oligo anti-miRNA-219a-5p with Xmotif |
AXMIR-219 |
SBI |
10 reactions |
EUR 438 |
|
ViraBind Adenovirus Miniprep Kit |
VPK-099 |
Cell Biolabs |
10 preps |
EUR 612 |
Description: Purification of viruses via cesium chloride ultracentrifugation procedures can be tedious and time-consuming. ViraBind Adenovirus Purification Kits provide a much more efficient system for fast adenoviral purification with high yields. The ViraBind Adenovirus Miniprep Kit uses a special spin column to purify supernatant from a single T75 flask or 10cm dish per prep in about 30 minutes. |
QuickTiter Adenovirus Quantitation Kit |
VPK-106 |
Cell Biolabs |
20 assays |
EUR 711 |
Description: Quantifying your adenovirus prep prior to infection will help you determine the amount of virus needed to achieve the appropriate MOI (multiplicity of infection) for your target cells. Our QuickTiter Adenovirus Quantitation Kit provides a quick method for measuring the viral nucleic acid content of your adenovirus. This assay may be performed either before or after purification of your virus. |
QuickTiter Lentivirus Titer Kit (Lentivirus-Associated p24 ELISA) |
VPK-107 |
Cell Biolabs |
96 assays |
EUR 775 |
Description: Measuring the HIV-1 p24 antigen is a long-established method for lentivirus quantitation. However, the traditional p24 ELISA detects both virus-associated p24 and free p24 generated by 293 cells during transient transfection. Free p24 can account for a substantial portion of total p24 in the supernatant. Therefore, the ELISA typically overestimates the quantity of lentivirus present. Our QuickTiter Lentivirus Titer Kit (Lentivirus-Associated HIV p24) substantially minimizes this problem. A proprietary technology separates the lentivirus from free p24 in solution prior to running the ELISA portion of the assay. |
QuickTiter Adenovirus Titer Immunoassay Kit |
VPK-109 |
Cell Biolabs |
100 assays |
EUR 705 |
Description: Accurate measurement of adenovirus titer is critical for gene delivery. Traditional plaque-forming unit (PFU) assays are long and suffer from high inter-assay variability. The QuickTiter Adenovirus Titer Immunoassay Kit provide a quick, complete system to functionally titer virus infectivity. The assay recognizes all 41 serotypes of adenovirus, and can be used with any adenovirus system that can amplify in HEK 293 cells. |
QuickTiter Adenovirus Titer ELISA Kit |
VPK-110 |
Cell Biolabs |
2 x 96 assays |
EUR 699 |
Description: Accurate measurement of adenovirus titer is critical for gene delivery. Traditional plaque-forming unit (PFU) assays are long and suffer from high inter-assay variability. The QuickTiter Adenovirus Titer ELISA Kit provides a quick, complete system to functionally titer virus infectivity. The assay recognizes all 41 serotypes of adenovirus, and can be used with any adenovirus system that can amplify in HEK 293 cells. |
Rapid RCA Assay Kit |
VPK-111 |
Cell Biolabs |
30 assays |
EUR 537 |
Description: Traditionally RCA (replication competent adenovirus) is measured in permissive cells by a plaque-forming unit (PFU) assay which takes 10-14 days. Our Rapid RCA Assay Kit uses an immunocytochemistry staining protocol that requires only a two day incubation. |
QuickTiter Lentivirus Quantitation Kit |
VPK-112 |
Cell Biolabs |
20 assays |
EUR 711 |
Description: It is often useful to quickly quantify your lentivirus prior to purification. Our QuickTiter Lentivirus Quantitation Kit provides a quick method for measuring the viral nucleic acid content of your lentivirus. This assay may be performed either before or after purification of your virus. |
QuickTiter Retrovirus Quantitation Kit |
VPK-120 |
Cell Biolabs |
20 assays |
EUR 711 |
Description: Our QuickTiter Retrovirus Quantitation Kit provides a quick method for determining retroviral titer. This assay measures the viral nucleic acid content of your retrovirus, and may be performed either before or after purification of your virus. |
ViraBind AAV Purification Kit |
VPK-140 |
Cell Biolabs |
10 preps |
EUR 711 |
Description: Purification of adeno-associated virus (AAV) using ultracentrifugation is tedious and time-consuming, and yields can be low. Our ViraBind AAV Purification Kits use a single-step proprietary purification matrix, followed by further purification and concentration with a centrifugal concentrator. The result is a higher viral yield with exceptional purity in a fraction of the time. |
ViraBind AAV Purification Mega Kit |
VPK-141 |
Cell Biolabs |
2 preps |
EUR 572 |
Description: Purification of adeno-associated virus (AAV) using ultracentrifugation is tedious and time-consuming, and yields can be low. Our ViraBind AAV Purification Kits use a single-step proprietary purification matrix, followed by further purification and concentration with a centrifugal concentrator. The result is a higher viral yield with exceptional purity in a fraction of the time. |
QuickTiter AAV Quantitation Kit |
VPK-145 |
Cell Biolabs |
20 assays |
EUR 711 |
Description: Traditionally, adeno-associated virus (AAV) particles have been quantified by DNA dot blot or similar procedures. These methods can be time-consuming and suffer from a high degree of inter-assay variability. Our QuickTiter AAV Quantitation Kit uses a proprietary technology to quantify the viral nucleic acid content of AAV preps. The kit can be used with unpurified supernatant of AAV-2 or AAV-DJ, or with purified AAV from any serotype. |
QuickTiter HBV Core Antigen ELISA Kit |
VPK-150 |
Cell Biolabs |
96 assays |
EUR 914 |
Description: An anti-HBVcAg monoclonal coating antibody is adsorbed onto a microtiter plate. HBV core antigen present in the sample or standard binds to the antibodies adsorbed on the plate; a FITC-conjugated mouse anti-HBVcAg antibody is added and binds to the antigen captured by the first antibody. Following incubation and wash steps, a HRP-conjugated mouse anti-FITC antibody is added and binds to the FITC conjugated anti-HBVcAg. Following unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of HBV core antigen present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from recombinant HBV core antigen and sample concentration is then determined. |
QuickTiter HCV Core Antigen ELISA Kit |
VPK-151 |
Cell Biolabs |
96 assays |
EUR 914 |
Description: An anti-HCVcAg monoclonal coating antibody is adsorbed onto a microtiter plate. HCV core antigen present in the sample or standard binds to the antibodies adsorbed on the plate; a FITC-conjugated mouse anti-HCVcAg antibody is added and binds to the antigen captured by the first antibody. Following incubation and wash steps, a HRP-conjugated mouse anti-FITC antibody is added and binds to the FITC conjugated anti-HCVcAg. Following unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of HCV core antigen present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from recombinant HCV core antigen and sample concentration is then determined. |
QuickTiter MuLV Core Antigen ELISA Kit |
VPK-156 |
Cell Biolabs |
96 assays |
EUR 914 |
Description: An anti-MuLV p30 monoclonal coating antibody is adsorbed onto a microtiter plate. MuLV core antigen (p30) present in the sample or standard binds to the antibodies adsorbed on the plate; an anti-MuLV p30 polyclonal antibody is added and binds to the antigen captured by the first antibody. Following incubation and wash steps, a HRP-conjugated secondary antibody is added and binds to the anti-MuLV p30 polyclonal. Unbound HRP-conjugated secondary antibody is removed during the wash steps, and substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of MuLV core antigen present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from recombinant MuLV p30 core antigen and sample concentration is then determined. |
ViraSafe Lentiviral Packaging System, Ecotropic |
VPK-205 |
Cell Biolabs |
1 kit |
EUR 891 |
Description: Our ViraSafe Lentiviral Packaging Systems contain three packaging plasmids for co-transfection with your own third generation lentiviral expression construct. If you need an expression vector as well as the packaging plasmids, consider our ViraSafe Lentiviral Expression Complete Systems. Our Ecotropic Packaging System assembles lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedure |