Elisa Assay With 2 Antibodies

Lab Reagents

Antibody Elisa Laboratories manufactures the elisa assay with 2 antibodies reagents distributed by Genprice. The Elisa Assay With 2 Antibodies reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact antibody elisa. Other Elisa products are available in stock. Specificity: Elisa Category: Assay Group: With 2

With 2 information

HDAC6 Fluorogenic Assay Kit

50076-2 384 rxns.
EUR 900
Description: The Fluorogenic HDAC6 Assay Kit is a complete assay system_x000D_ designed to measure histone deacetylase 6 (HDAC6) activity for screening and profiling_x000D_ applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_ 100 fluorescent HDAC6 activity measurements. In addition, the kit includes purified_x000D_HDAC6 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_ negative control. The Fluorogenic HDAC6 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_ radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC activity_x000D_ level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_ is incubated with purified HDAC enzyme. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_ be measured using a fluorescence reader.

SetDB1 Chemiluminescent Assay Kit

51056-2 384 rxns.
EUR 1145
Description: The SetDB1 Chemiluminescent Assay Kit is designed to measure SetDB1 activity for screening and profiling applications. The advantage of the SetDB1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes the methylated K9 residue of Histone H3. With this kit, only three simple steps are required. First, S-adenosylmethionine is incubated with a sample containing the assay buffer and the methyltransferase enzyme. Next, the primary antibody is added. Finally, the wells are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

RANK:RANKL TR-FRET Assay

79101-2 384 rxns.
EUR 1095
Description: The RANK:RANKL TR-FRET Assay is designed to measure the inhibition of RANK binding to RANKL in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated RANK, RANKL, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.

PARP7 Chemiluminescent Assay Kit

79729-2 384 rxns.
EUR 2305
Description: The PARP7 Chemiluminescent Activity Assay Kit is designed to measure PARP7 activity for screening and profiling applications. PARP7 is known to catalyze the NAD-dependent addition of poly (ADP-ribose) to histones. With this kit, only three simple steps are required for PARP7 reactions. First, histone proteins are coated on a 384-well plate. Next, the PARP7 biotinylated substrate is incubated with an assay buffer that contains the PARP7 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

PARP2 Chemiluminescent Assay Kit

80552-2 384 rxns.
EUR 1340
Description: The PARP2 Chemiluminescent Assay Kit is designed to measure PARP2 activity for screening and profiling applications. PARP2 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP2 Chemiluminescent Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for PARP2 reactions. First, histone proteins are coated on a 96-well or 384-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP2 enzyme and an activated DNA template in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.*NOTE: As of March 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.

CytoX-Violet Cell Proliferation/Cytotoxicity Assay Kit 

OP-0005
  • EUR 474.12
  • EUR 138.60
  • EUR 189.20
  • EUR 292.60
  • 20x96 assays
  • 5x96 assays
  • 10x96 assays
  • 20x96 assays

Custom production of antibodies in 2 Chickens using supplied antigen (std 63 days protocol)

CHI-2 1
EUR 1365.6

Custom production of antibodies in 2 Rabbits using supplied antigen (std 63 days protocol)

RAB-2 1
EUR 1365.6

Papain-like Protease (SARS-CoV-2) Assay Kit: Protease Activity

79995-2 384 rxns.
EUR 1240
Description: The Papain-like Protease Assay Kit: Protease Activity is designed to measure PLPro activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.

EpiQuik Superoxide Dismutase Activity/Inhibition Assay Kit (Colorimetric) 

OP-0001
  • EUR 595.92
  • EUR 121.00
  • EUR 213.40
  • EUR 380.60
  • 5x96 assays
  • 96 Assays
  • 2x96 assays
  • 5x96 assays

Custom production of antibodies in 2 G. Pigs using supplied antigen (std 63 days protocol)

GPI-2 1
EUR 1365.6

Rac2 Activation Assay

STA-401-2 20 assays
EUR 908.4
Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit.

3CL Protease (B.1.1.529, Omicron Variant) (SARS-CoV-2) Assay Kit

78350-2 384 rxns.
EUR 1315
Description: The 3CL Protease (B.1.1.529, Omicron Variant) (SARS-CoV-2) Assay Kit is designed to measure the activity of P132H mutated, Omicron variant 3CL Protease for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.The 3CL Protease Substrate is an internally quenched 14-mer fluorogenic (FRET) peptide (DABCYL-KTSAVLQSGFRKME-EDANS). When the donor (EDANS) and acceptor (DABCYL) fluorophores are in close proximity, the energy emitted from EDANS is quenched by DABCYL (intact substrate). Upon proteolysis by 3CL, the peptide substrate is cleaved between glutamine and serine to generate the highly fluorescent peptide fragment (SGFRKME-EDANS). The fluorescence intensity increases proportionally to the activity of 3CL. More information on the substrate, including MW and structure, can be found on our website (BPS Bioscience, #79952).

Spike S1-Biotin (SARS-CoV-2): ACE2 TR-FRET Assay Kit

79949-2 384 rxns.
EUR 1265
Description: The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer some protection against the viral infection._x000D_The SARS-CoV-2 Spike S1:ACE2 TR-FRET Assay is designed to measure the inhibition of the binding between SARS-CoV-2 Spike S1 and human ACE2 in a homogeneous 96 or 384 reaction format. This TR-FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; the test inhibitor compound is incubated with biotinylated Spike S1, Eu-labeled ACE2, dye-labeled acceptor and an inhibitor for one hour. Then the TR-FRET signal is measured using a fluorescence reader._x000D_ 

Firefly Luciferase Assay Kit (Lyophilized)

30075-2 1000TST
EUR 550.8
Description: Minimum order quantity: 1 unit of 1000TST

Renilla Luciferase Assay Kit 2.0

30082-2 1000AS
EUR 712.8
Description: Minimum order quantity: 1 unit of 1000AS

Firefly Luciferase Assay Kit 2.0

30085-2 1000AS
EUR 550.8
Description: Minimum order quantity: 1 unit of 1000AS