Oxiselect In Vitro Ros Assay

OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence)
STA-347	Cell Biolabs	96 assays	EUR 734.4
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.

Human IgG antibody Laboratories manufactures the oxiselect in vitro ros assay reagents distributed by Genprice. The Oxiselect In Vitro Ros Assay reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact Oxiselect. Other Oxiselect products are available in stock. Specificity: Oxiselect Category: In Group: Vitro Ros

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence)
Cell Biolabs	96 assays	EUR 762
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence)
Cell Biolabs	5 x 96 assays	EUR 3058.8
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

CytoSelect 96-well In Vitro Tumor Sensitivity Assay (Soft Agar Colony Formation)
Cell Biolabs	96 assays	EUR 922.8
Description: Traditionally, the soft agar colony formation assay has been used to monitor anchorage-independent growth. Cells proliferate for 3-4 weeks in a semisolid culture medium, followed by tedious manual counting of colonies. Our CytoSelect 96-Well In Vitro Tumor Sensitivity Assay provides a stringent, anchorage-independent model for chemosenstivity testing and screening of potential anticancer drugs. After just 6-8 days, cell colonies are quantified using a standard colorimetric microplate reader.

CytoSelect 96-well In Vitro Tumor Sensitivity Assay (Soft Agar Colony Formation)
Cell Biolabs	5 x 96 assays	EUR 3811.2
Description: Traditionally, the soft agar colony formation assay has been used to monitor anchorage-independent growth. Cells proliferate for 3-4 weeks in a semisolid culture medium, followed by tedious manual counting of colonies. Our CytoSelect 96-Well In Vitro Tumor Sensitivity Assay provides a stringent, anchorage-independent model for chemosenstivity testing and screening of potential anticancer drugs. After just 6-8 days, cell colonies are quantified using a standard colorimetric microplate reader.

ROS antibody
Fitzgerald	100 ul	EUR 344.4
Description: Purified Polyclonal ROS antibody

ROS Antibody
Affbiotech	200ul	EUR 420

Antibody for Human ROS
Stressmarq	0.1ml	EUR 424.8
Description: A polyclonal antibody for ROS from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of human ROS (AA1924-1938). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This ROS antibody is unconjugated.

Vitro Ros information

OxiSelect Comet Assay Slides (3-Well)
STA-353-5	Cell Biolabs	5 x 25 slides	EUR 1521.6
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

OxiSelect 96-Well Comet Assay Slide
STA-356	Cell Biolabs	1 slide	EUR 219.6
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

OxiSelect 96-Well Comet Assay Slide
STA-356-5	Cell Biolabs	5 slides	EUR 622.8
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

OxiSelect Glutathione Reductase Assay Kit
STA-812	Cell Biolabs	100 assays	EUR 505.2
Description: Cell Biolabs? OxiSelect Glutathione Reductase Assay Kit is a   Glutathione reductase activity is defined as 1 unit of enzyme reducing 1 µmole oxidized glutathione (GSSG) per minute at pH 7.6 and 25ºC.  The kit employs a simple enzymatic recycling reaction for glutathione quantification where the reduction of a chromagen is correlated to glutathione reductase enzymatic activity.  The kit has a detection sensitivity limit of approximately 0.6 mU/mL.  Each kit provides sufficient reagents to perform up to 100 assays, including standard curve and unknown samples.

OxiSelect Ascorbic Acid Assay Kit (FRASC)
STA-860	Cell Biolabs	200 assays	EUR 672
Description: Ascorbic acid is a vital water-soluble antioxidant found in living organisms. Ascorbic acid is critical for a variety of functions related to tissue growth and wound healing, neurotransmitter formation, blood cholesterol levels, as well as free radical neutralization. The OxiSelect Ascorbic Acid Assay Kit is a quantitative assay for measuring ascorbic acid within various samples. The assay is based on the Ferric Reducing/Antioxidant Ascorbic Acid (FRASC) chemistry driven by the electron donating reducing power of antioxidants. The kit employs ascorbate oxidase, which allows the user to differentiate the ascorbic acid content from other antioxidants present within their sample. Ascorbic acid levels in a sample are determined by measuring the difference in optical density between two sample wells, one with and one without the enzyme.

OxiSelect TBARS Assay Kit (MDA Quantitation)
STA-330	Cell Biolabs	200 assays	EUR 790.8
Description: The TBARS (Thiobarbituric Acid Reactive Substances) assay is well-established for screening and monitoring lipid peroxidation. MDA forms a 1:2 adduct with thiobarbituric acid; the MDA-TBA adduct can then be measured. Our OxiSelect TBARS Assay Kit provides a much more user-friendly protocol to measure the MDA-TBA adduct. Reaction volumes are much smaller than the traditional assay, so much less sample is required. Also, reactions can be performed in standard polypropylene tubes - no glass tubes or glass marbles are required. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE.

OxiSelect TBARS Assay Kit (MDA Quantitation)
STA-330-5	Cell Biolabs	5 x 200 assays	EUR 3205.2
Description: The TBARS (Thiobarbituric Acid Reactive Substances) assay is well-established for screening and monitoring lipid peroxidation. MDA forms a 1:2 adduct with thiobarbituric acid; the MDA-TBA adduct can then be measured. Our OxiSelect TBARS Assay Kit provides a much more user-friendly protocol to measure the MDA-TBA adduct. Reaction volumes are much smaller than the traditional assay, so much less sample is required. Also, reactions can be performed in standard polypropylene tubes - no glass tubes or glass marbles are required. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE.

OxiSelect Protein Carbonyl Fluorometric Assay
STA-307	Cell Biolabs	100 assays	EUR 783.6
Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl Fluorometric Assay Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The fluorescence plate-based format provides a convenient system for direct measurement of protein carbonyl content.

OxiSelect Comet Assay Kit (3-Well Slides)
STA-350	Cell Biolabs	15 assays	EUR 393.6
Description: Our OxiSelect 3-Well Comet Assay Kits provide a fast, convenient way to screen for general DNA damage, regardless of the source or nature of the damage. Kits include Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

OxiSelect Comet Assay Kit (3-Well Slides)
STA-351	Cell Biolabs	75 assays	EUR 721.2
Description: Our OxiSelect 3-Well Comet Assay Kits provide a fast, convenient way to screen for general DNA damage, regardless of the source or nature of the damage. Kits include Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

OxiSelect Comet Assay Kit (3-Well Slides)
STA-351-5	Cell Biolabs	5 x 75 assays	EUR 2836.8
Description: Our OxiSelect 3-Well Comet Assay Kits provide a fast, convenient way to screen for general DNA damage, regardless of the source or nature of the damage. Kits include Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

OxiSelect Free Hydrogen Sulfide Gas Assay Kit
XAN-5084	Cell Biolabs	100 assays	EUR 512.4

OxiSelect Total Glutathione (GSSG/GSH) Assay Kit
STA-312	Cell Biolabs	100 assays	EUR 622.8
Description: The OxiSelect Total Glutathione Assay Kit is a quantitative assay for measuring the total glutathione content within a sample (GSH/GSSG).  Glutathione Reductase reduces oxidized glutathione (GSSG) to reduced glutathione (GSH) in the presence of NADPH.  Subsequently, the  chromogen reacts with the thiol group of GSH to produce a colored compound that absorbs at 405 nm.  The total glutathione content in unknown samples is determined by comparison with the predetermined glutathione standard curve.  The rate of chromophore production is proportional to the concentration of glutathione within the sample.  The rate can be determined from the absorbance change over time.  Metaphosphoric acid is provided to remove interfering proteins or enzymes from samples.

OxiSelect Superoxide Dismutase Activity Assay Kit
STA-340	Cell Biolabs	100 assays	EUR 762
Description: Superoxide dismutase (SOD), which catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen, is one of the most important antioxidant enzymes. SOD enzymes are classified into three groups: cytosolic CuZn-SOD, mitochondrial Mn-SOD, and extracellular Ec-SOD. Our OxiSelect Superoxide Dismutase Activity Assay uses a xanthine/xanthine oxidase (XOD) system to generate superoxide anions and a chromagen to produce a water-soluble formazan dye upon reduction by superoxide anions. The superoxide dismutase activity is determined as the inhibition or reduction of chromagen.

OxiSelect Monoamine Oxidase Assay Kit (Fluorometric)
XPX-5000	Cell Biolabs	96 assays	EUR 540
Description: Our Monoamine Oxidase Assay Kit measures MOA-A and MAO-B in biological samples. MAO reacts with a substrate, generating hydrogen peroxide. A reaction between hydrogen peroxide and the probe results in a signal that is directly proportional to the level of MAO in the sample and is detected fluorescence plate reader. MAO activity in unknown samples is calculated based on a hydrogen peroxide standard curve.

OxiSelect Monoamine Oxidase Assay Kit (Fluorometric)
XPX-5000-5	Cell Biolabs	5 x 96 assays	EUR 2154
Description: Our Monoamine Oxidase Assay Kit measures MOA-A and MAO-B in biological samples. MAO reacts with a substrate, generating hydrogen peroxide. A reaction between hydrogen peroxide and the probe results in a signal that is directly proportional to the level of MAO in the sample and is detected fluorescence plate reader. MAO activity in unknown samples is calculated based on a hydrogen peroxide standard curve.

OxiSelect Monoamine Oxidase Assay Kit (Colorimetric)
XPX-5006	Cell Biolabs	96 assays	EUR 540
Description: Our Monoamine Oxidase Assay Kit measures MOA-A and MAO-B in biological samples. MAO reacts with a substrate, generating hydrogen peroxide. A reaction between hydrogen peroxide and the probe results in a signal that is directly proportional to the level of MAO in the sample and is detected colorimetric plate reader. MAO activity in unknown samples is calculated based on a hydrogen peroxide standard curve.