Quantitative and Qualitative QC of Next-Generation Sequencing for Detecting Somatic Variants: An Example of Detecting Clonal Hematopoiesis of Indeterminate Potential.

Because generation sequencing (NGS) to detect somatic mutations have been adopted in the clinical field, both qualitative and quantitative QC of somatic variants through the entire coding region was detected by NGS are very important. However, application or specific guidelines, especially for quantitative QC, currently insufficient.

Our goal is to design a practical approach to both quantitative and qualitative QC using the example of detecting clonal hematopoiesis indefinite potential (CHIP) .We implement a QC scheme using commercial materials and in-house reference materials QC (IQCM) consists of a map of haplotypes and cell lines cancer to monitor CHIP.This efficient approach validated assay adapted CHIP NGS.

Accuracy, analytical sensitivity, analytical specificity, precision qualitative (concordance), and the detection limits were reached 99.87%, 98.53%, 100.00%, 100.00%, and 1.00%, respectively. Analysis of quantitative precision CV also has a higher percentage in the lower depths of alternative read (R2 = 0749 ~ 0858). The use IQCM ensure a reduction of more than 100-fold in the cost per run compared to that achieved using a commercial approach defined reference materials.

Our common analytical performance NGS to detect CHIP and limitations are recognized as lower precision on the lower level of the load variants. This approach could also theoretically be extended to the general NGS assay for the detection of somatic variants. Given NGS reliable results and cost-effectiveness, we propose the use of NGS IQCM for QC tests in clinical laboratories.

Given the potential evidence of the real world (RWE) to inform understand the risk profile benefit from next-generation sequencing (NGS) based testing, we conducted a study to describe the landscape at this time whether and how the payer uses RWE as part of the decision-making scope and potential solutions to overcome barriers. We conducted a literature review of evidence scoping RWE existing framework to evaluate new technologies and identified barriers to clinical integration and evidence gaps for NGS. We synthesized the findings as a potential solution to improve the relevance and utility RWE for payer decision-making.

TRXR2 antibody

70R-30818 100 ug
EUR 327
Description: Rabbit polyclonal TRXR2 antibody

TrxR2 Polyclonal Antibody

ES3647-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against TrxR2 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

TrxR2 Polyclonal Antibody

ES3647-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against TrxR2 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

TrxR2 Polyclonal Antibody

ABP52648-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of TrxR2 from Human, Mouse, Rat. This TrxR2 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human TrxR2 at AA range: 440-520

TrxR2 Polyclonal Antibody

ABP52648-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of TrxR2 from Human, Mouse, Rat. This TrxR2 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human TrxR2 at AA range: 440-520

TrxR2 Polyclonal Antibody

ABP52648-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of TrxR2 from Human, Mouse, Rat. This TrxR2 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human TrxR2 at AA range: 440-520

Anti-TRXR2 Antibody

A03900 100ul
EUR 397
Description: Rabbit Polyclonal TRXR2 Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.

Anti-TrxR2 antibody

STJ96117 200 µl
EUR 197
Description: Rabbit polyclonal to TrxR2.

Polyclonal TRXR2 Antibody

APR10548G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human TRXR2 . This antibody is tested and proven to work in the following applications:

TRXR2 Conjugated Antibody

C33530 100ul
EUR 397

TRXR2 Blocking Peptide

AF5399-BP 1mg
EUR 195

TRXR2 Cell ELISA Kit

abx595605-96tests 96 tests
EUR 637

Thioredoxin Reductase 2 (TRXR2) Antibody

20-abx013238
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Thioredoxin Reductase 2 (TRXR2) Antibody

abx415336-50ug 50 ug
EUR 565

TRXR2 ELISA Kit (Human) (OKCD04198)

OKCD04198 96 Wells
EUR 831
Description: Description of target: The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes a mitochondrial form important for scavenging reactive oxygen species in mitochondria. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms, including a few localized in the cytosol and some lacking the C-terminal Sec residue, have been found for this gene.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.124 ng/mL

TRXR2 Colorimetric Cell-Based ELISA Kit

EKC1577 100ul
EUR 572

Recombinant Plasmodium Falciparum trxr2 Protein (Baculovirus)

VAng-Wyb6880-inquire inquire Ask for price
Description: Plasmodium Falciparum (isolate 3D7) Thioredoxin reductase 2, partial, recombinant protein.

Recombinant Plasmodium Falciparum trxr2 Protein (Yeast)

VAng-Wyb6878-inquire inquire Ask for price
Description: Plasmodium Falciparum (isolate 3D7) Thioredoxin reductase 2, partial, recombinant protein.

Human Thioredoxin Reductase 2 ELISA Kit (TrxR2)

RK02454 96 Tests
EUR 521

Recombinant Plasmodium Falciparum trxr2 Protein (E. coli)

VAng-Wyb6879-inquire inquire Ask for price
Description: Plasmodium Falciparum (isolate 3D7) Thioredoxin reductase 2, partial, recombinant protein.

Recombinant Plasmodium Falciparum trxr2 Protein (Mammalian cel)

VAng-Wyb6881-inquire inquire Ask for price
Description: Plasmodium Falciparum (isolate 3D7) Thioredoxin reductase 2, partial, recombinant protein.

Human Thioredoxin Reductase 2 (TrxR2) CLIA Kit

20-abx494240
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Thioredoxin Reductase 2 (TrxR2) ELISA Kit

SED376Hu-10x96wellstestplate 10x96-wells test plate
EUR 4731.5
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Thioredoxin Reductase 2 (TrxR2) in Tissue homogenates, cell lysates and other biological fluids.

Human Thioredoxin Reductase 2 (TrxR2) ELISA Kit

SED376Hu-1x48wellstestplate 1x48-wells test plate
EUR 477.3
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Thioredoxin Reductase 2 (TrxR2) in Tissue homogenates, cell lysates and other biological fluids.

Human Thioredoxin Reductase 2 (TrxR2) ELISA Kit

SED376Hu-1x96wellstestplate 1x96-wells test plate
EUR 639
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Thioredoxin Reductase 2 (TrxR2) in Tissue homogenates, cell lysates and other biological fluids.

Human Thioredoxin Reductase 2 (TrxR2) ELISA Kit

SED376Hu-5x96wellstestplate 5x96-wells test plate
EUR 2575.5
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Thioredoxin Reductase 2 (TrxR2) in Tissue homogenates, cell lysates and other biological fluids.

Human Thioredoxin Reductase 2 (TrxR2) ELISA Kit

4-SED376Hu
  • EUR 4782.00
  • EUR 2526.00
  • EUR 640.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Thioredoxin Reductase 2 (TrxR2) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Thioredoxin Reductase 2(TrxR2)ELISA Kit

QY-E02233 96T
EUR 361

TRXR2 Colorimetric Cell-Based ELISA Kit (OKAG01094)

OKAG01094 96 Wells
EUR 596
Description: Description of target: ;Species reactivity: Human, Mouse, Rat;Application: ELISA;Assay info: Assay Type: Cell-Based
Subtype: None
Detection Method: Colorimetric 450 nm;Sensitivity:

ELISA kit for Human TrxR2 (Thioredoxin Reductase 2)

ELK5334 1 plate of 96 wells
EUR 432
Description: A sandwich ELISA kit for detection of Thioredoxin Reductase 2 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Recombinant Plasmodium Falciparum trxr2 Protein (aa 1-617)

VAng-Wyb6877-inquire inquire Ask for price
Description: Plasmodium Falciparum (isolate 3D7) Thioredoxin reductase 2, partial, recombinant protein.