Quantitative and Qualitative QC of Next-Generation Sequencing for Detecting Somatic Variants: An Example of Detecting Clonal Hematopoiesis of Indeterminate Potential.

Because generation sequencing (NGS) to detect somatic mutations have been adopted in the clinical field, both qualitative and quantitative QC of somatic variants through the entire coding region was detected by NGS are very important. However, application or specific guidelines, especially for quantitative QC, currently insufficient.

Our goal is to design a practical approach to both quantitative and qualitative QC using the example of detecting clonal hematopoiesis indefinite potential (CHIP) .We implement a QC scheme using commercial materials and in-house reference materials QC (IQCM) consists of a map of haplotypes and cell lines cancer to monitor CHIP.This efficient approach validated assay adapted CHIP NGS.

Accuracy, analytical sensitivity, analytical specificity, precision qualitative (concordance), and the detection limits were reached 99.87%, 98.53%, 100.00%, 100.00%, and 1.00%, respectively. Analysis of quantitative precision CV also has a higher percentage in the lower depths of alternative read (R2 = 0749 ~ 0858). The use IQCM ensure a reduction of more than 100-fold in the cost per run compared to that achieved using a commercial approach defined reference materials.

Our common analytical performance NGS to detect CHIP and limitations are recognized as lower precision on the lower level of the load variants. This approach could also theoretically be extended to the general NGS assay for the detection of somatic variants. Given NGS reliable results and cost-effectiveness, we propose the use of NGS IQCM for QC tests in clinical laboratories.

Given the potential evidence of the real world (RWE) to inform understand the risk profile benefit from next-generation sequencing (NGS) based testing, we conducted a study to describe the landscape at this time whether and how the payer uses RWE as part of the decision-making scope and potential solutions to overcome barriers. We conducted a literature review of evidence scoping RWE existing framework to evaluate new technologies and identified barriers to clinical integration and evidence gaps for NGS. We synthesized the findings as a potential solution to improve the relevance and utility RWE for payer decision-making.

Anti-TRXR2 Antibody
A03900 100ul
EUR 476.4
Description: Rabbit Polyclonal TRXR2 Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.

TrxR2 Polyclonal Antibody
ABP52648-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of TrxR2 from Human, Mouse, Rat. This TrxR2 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human TrxR2 at AA range: 440-520

TrxR2 Polyclonal Antibody
ABP52648-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of TrxR2 from Human, Mouse, Rat. This TrxR2 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human TrxR2 at AA range: 440-520

TrxR2 Polyclonal Antibody
ABP52648-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of TrxR2 from Human, Mouse, Rat. This TrxR2 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human TrxR2 at AA range: 440-520

Polyclonal TRXR2 Antibody
APR10548G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human TRXR2 . This antibody is tested and proven to work in the following applications:

TRXR2 Conjugated Antibody
C33530 100ul
EUR 476.4

TRXR2 Blocking Peptide
AF5399-BP 1mg
EUR 234

TrxR2 Polyclonal Antibody
ES3647-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against TrxR2 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

TrxR2 Polyclonal Antibody
ES3647-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against TrxR2 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

Anti-TrxR2 antibody
STJ96117 200 µl
EUR 236.4
Description: Rabbit polyclonal to TrxR2.

TRXR2 Cell ELISA Kit
abx595605-96tests 96 tests
EUR 764.4

Thioredoxin Reductase 2 (TRXR2) Antibody
20-abx013238
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Thioredoxin Reductase 2 (TRXR2) Antibody
abx415336-50ug 50 ug
EUR 678

TRXR2 ELISA Kit (Human) (OKCD04198)
OKCD04198 96 Wells
EUR 997.2
Description: Description of target: The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes a mitochondrial form important for scavenging reactive oxygen species in mitochondria. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms, including a few localized in the cytosol and some lacking the C-terminal Sec residue, have been found for this gene.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.124 ng/mL

TRXR2 Colorimetric Cell-Based ELISA Kit
EKC1577 100ul
EUR 686.4

Recombinant Plasmodium Falciparum trxr2 Protein (Yeast)
VAng-Wyb6878-inquire inquire Ask for price
Description: Plasmodium Falciparum (isolate 3D7) Thioredoxin reductase 2, partial, recombinant protein.

Recombinant Plasmodium Falciparum trxr2 Protein (Baculovirus)
VAng-Wyb6880-inquire inquire Ask for price
Description: Plasmodium Falciparum (isolate 3D7) Thioredoxin reductase 2, partial, recombinant protein.

Human Thioredoxin Reductase 2 (TrxR2) CLIA Kit
20-abx494240
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

TRXR2 Colorimetric Cell-Based ELISA Kit (OKAG01094)
OKAG01094 96 Wells
EUR 715.2
Description: Description of target: ;Species reactivity: Human, Mouse, Rat;Application: ELISA;Assay info: Assay Type: Cell-Based
Subtype: None
Detection Method: Colorimetric 450 nm;Sensitivity:

Human Thioredoxin Reductase 2 (TrxR2) ELISA Kit
SED376Hu-10x96wellstestplate 10x96-wells test plate
EUR 5677.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Thioredoxin Reductase 2 (TrxR2) in Tissue homogenates, cell lysates and other biological fluids.

Human Thioredoxin Reductase 2 (TrxR2) ELISA Kit
SED376Hu-1x48wellstestplate 1x48-wells test plate
EUR 572.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Thioredoxin Reductase 2 (TrxR2) in Tissue homogenates, cell lysates and other biological fluids.

Human Thioredoxin Reductase 2 (TrxR2) ELISA Kit
SED376Hu-1x96wellstestplate 1x96-wells test plate
EUR 766.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Thioredoxin Reductase 2 (TrxR2) in Tissue homogenates, cell lysates and other biological fluids.

Human Thioredoxin Reductase 2 (TrxR2) ELISA Kit
SED376Hu-5x96wellstestplate 5x96-wells test plate
EUR 3090.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Thioredoxin Reductase 2 (TrxR2) in Tissue homogenates, cell lysates and other biological fluids.

Human Thioredoxin Reductase 2 (TrxR2) ELISA Kit
4-SED376Hu
  • EUR 5738.40
  • EUR 3031.20
  • EUR 768.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Thioredoxin Reductase 2 (TrxR2) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Thioredoxin Reductase 2(TrxR2)ELISA Kit
QY-E02233 96T
EUR 433.2

Human Thioredoxin Reductase 2 ELISA Kit (TrxR2)
RK02454 96 Tests
EUR 625.2

Recombinant Plasmodium Falciparum trxr2 Protein (E. coli)
VAng-Wyb6879-inquire inquire Ask for price
Description: Plasmodium Falciparum (isolate 3D7) Thioredoxin reductase 2, partial, recombinant protein.

Recombinant Plasmodium Falciparum trxr2 Protein (Mammalian cel)
VAng-Wyb6881-inquire inquire Ask for price
Description: Plasmodium Falciparum (isolate 3D7) Thioredoxin reductase 2, partial, recombinant protein.

ELISA kit for Human TrxR2 (Thioredoxin Reductase 2)
ELK5334 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Thioredoxin Reductase 2 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Recombinant Plasmodium Falciparum trxr2 Protein (aa 1-617)
VAng-Wyb6877-inquire inquire Ask for price
Description: Plasmodium Falciparum (isolate 3D7) Thioredoxin reductase 2, partial, recombinant protein.