Development and validation of next generation sequencing based 35-gene hereditary cancer panel.

Understanding the genetic basis of cancer risk is an international effort. The advent of next-generation sequencing (NGS) in late 2000 has further accelerated the discovery of many cancer susceptibility genes. The use of targeted NGS-panel multigene testing to provide a comprehensive analysis of cancer susceptibility genes has proven to be a viable option, with accurate and robust detection of clinically relevant variants in targeted gene into crucial.

We has been developed and validated tests are targeted based NGS for risk assessment of hereditary cancer using Illumina NGS Platform by analyzing the protein-coding regions of 35 genes hereditary cancer with bioinformatics pipeline that utilize standard practice in the field.

35-offspring cancer panel is designed to identify the gene germline mutations cause cancer to 8 different cancers: breast, ovary, prostate, uterus, colon, pancreatic, gastric cancer and melanoma. Panel validated using DNA specimens were characterized [NIGMS Human Genetic Cell Repository], where the DNA was extracted using the blood of individual genetic variants had previously been characterized by the Genome Project in 1000 and Coriell Catalog.

The 35-gene panel hereditary cancer showed high sensitivity (99.9%) and specificity (100%) in 4820 variants including single nucleotide variants (SNVs) and small insertions and deletions (INDEL; up to 25 bp). Reproducibility and repeatability is 99.8 and 100%, respectively.The use of NGS targeted based multigene test panel to provide a comprehensive analysis of cancer susceptibility genes has been considered as a viable option.

In this study, we developed and validated a 35-gene panel for testing eight common cancers using next-generation sequencing (NGS). Our descent cancer panel performance rated on the board of numerous variants in 35 genes to support the clinical use.

Understanding the genetic basis of cancer risk is an international effort. The advent of next-generation sequencing (NGS) in late 2000 has further accelerated the discovery of many cancer susceptibility genes. The use of targeted NGS-panel multigene testing to provide a comprehensive analysis of cancer susceptibility genes has proven to be a viable option, with accurate and robust detection of clinically relevant variants in targeted gene into crucial.We has been developed and validated tests are targeted based NGS for risk assessment of hereditary cancer using Illumina NGS Platform by analyzing the protein-coding regions of 35 genes hereditary cancer with bioinformatics pipeline that utilize standard practice in the field. 35-offspring cancer panel is designed to identify the gene germline mutations cause cancer to 8 different cancers: breast, ovary, prostate, uterus, colon, pancreatic, gastric cancer and melanoma. Panel validated using DNA specimens were characterized [NIGMS Human Genetic Cell Repository], where the DNA was extracted using the blood of individual genetic variants had previously been characterized by the Genome Project in 1000 and Coriell Catalog.The 35-gene panel hereditary cancer showed high sensitivity (99.9%) and specificity (100%) in 4820 variants including single nucleotide variants (SNVs) and small insertions and deletions (INDEL; up to 25 bp). Reproducibility and repeatability is 99.8 and 100%, respectively.The use of NGS targeted based multigene test panel to provide a comprehensive analysis of cancer susceptibility genes has been considered as a viable option. In this study, we developed and validated a 35-gene panel for testing eight common cancers using next-generation sequencing (NGS). Our descent cancer panel performance rated on the board of numerous variants in 35 genes to support the clinical use.
Development and validation of next generation sequencing based 35-gene hereditary cancer panel.

TUBA8 Antibody

BF0558 200ul
EUR 376.00
Description: TUBA8 antibody detects endogenous levels of total TUBA8.

TUBA8 siRNA

20-abx938554
  • EUR 551.00
  • EUR 732.00
  • 0
  • 1
  • Shipped within 5-10 working days.

TUBA8 siRNA

20-abx938555
  • EUR 551.00
  • EUR 732.00
  • 0
  • 1
  • Shipped within 5-10 working days.

TUBA8 antibody

70R-21058 50 ul
EUR 435.00
Description: Rabbit polyclonal TUBA8 antibody

Tuba8 antibody

70R-9491 50 ug
EUR 467.00
Description: Affinity purified rabbit polyclonal Tuba8 antibody

TUBA8 antibody

70R-9492 50 ug
EUR 467.00
Description: Affinity purified rabbit polyclonal TUBA8 antibody

TUBA8 Antibody

48461-100ul 100ul
EUR 333.00

TUBA8 Antibody

48461-50ul 50ul
EUR 239.00

TUBA8 antibody

10R-6169 100 ul
EUR 691.00
Description: Mouse monoclonal TUBA8 antibody

TUBA8 antibody

10R-6170 100 ul
EUR 691.00
Description: Mouse monoclonal TUBA8 antibody

TUBA8 antibody

10R-6171 100 ul
EUR 691.00
Description: Mouse monoclonal TUBA8 antibody

TUBA8 antibody

10R-6172 100 ul
EUR 691.00
Description: Mouse monoclonal TUBA8 antibody

TUBA8 Antibody

1-CSB-PA025314GA01HU
  • EUR 597.00
  • EUR 333.00
  • 0
  • 1
  • Form: Liquid
  • Buffer: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. -20oC, Avoid freeze / thaw cycles. Antigen Affinity purified
Description: A polyclonal antibody against TUBA8. Recognizes TUBA8 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA

anti-TUBA8

YF-PA19219 100 ug
EUR 403.00
Description: Rabbit polyclonal to TUBA8

TUBA8 Conjugated Antibody

C48461 100ul
EUR 397.00

TUBA8 Blocking Peptide

BF0558-BP 1mg
EUR 195.00

Tuba8 Blocking Peptide

33R-1093 100 ug
EUR 180.00
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of CNTFR antibody, catalog no. 70R-9962

TUBA8 Blocking Peptide

33R-2653 100 ug
EUR 180.00
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of TUBA8 antibody, catalog no. 70R-9492

anti-TUBA8 (2D6)

LF-MA30664 100 ul
EUR 527.00
Description: Mouse Monoclonal to TUBA8

Mouse TUBA8 shRNA Plasmid

20-abx974377
  • EUR 801.00
  • EUR 1121.00
  • 0
  • 1
  • Shipped within 15-20 working days.

Human TUBA8 shRNA Plasmid

20-abx959952
  • EUR 801.00
  • EUR 1121.00
  • 0
  • 1
  • Shipped within 15-20 working days.

Rat TUBA8 shRNA Plasmid

20-abx990973
  • EUR 801.00
  • EUR 1121.00
  • 0
  • 1
  • Shipped within 15-20 working days.

TUBA8 Recombinant Protein (Human)

RP105950 100 ug Ask for price

TUBA8 Recombinant Protein (Rat)

RP235268 100 ug Ask for price

TUBA8 Recombinant Protein (Mouse)

RP182228 100 ug Ask for price

Monoclonal TUBA8 Antibody, Clone: 2D6

APR13863G 0.1ml
EUR 528.00
Description: A Monoclonal antibody against Human TUBA8. The antibodies are raised in Mouse and are from clone 2D6. This antibody is applicable in WB and IHC, FC, ICC, E

Tubulin, Alpha 8 (TUBA8) Antibody

20-abx116348
  • EUR 732.00
  • EUR 398.00
  • 0
  • 1
  • Shipped within 5-10 working days.

TUBA8 ORF Vector (Human) (pORF)

ORF035318 1.0 ug DNA
EUR 405.00

Tuba8 ORF Vector (Rat) (pORF)

ORF078424 1.0 ug DNA
EUR 506.00

Tuba8 ORF Vector (Mouse) (pORF)

ORF060744 1.0 ug DNA
EUR 506.00

Polyclonal Tuba8 antibody - N-terminal region

AMM08377G 0.05mg
EUR 528.00
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Tuba8 - N-terminal region. This antibody is tested and proven to work in the following applications:

Tubulin alpha 8 (TUBA8) polyclonal antibody

ABP-PAB-01423 100 ug Ask for price
    • Product line: Miscellaneous
    • Brand:

Tubulin Alpha-8 Chain (TUBA8) Antibody

abx011988-100ul 100 ul
EUR 411.00
  • Shipped within 5-10 working days.

TUBA8 sgRNA CRISPR Lentivector set (Human)

K2557901 3 x 1.0 ug
EUR 339.00

Tuba8 sgRNA CRISPR Lentivector set (Mouse)

K3429401 3 x 1.0 ug
EUR 339.00

Tuba8 sgRNA CRISPR Lentivector set (Rat)

K6710901 3 x 1.0 ug
EUR 339.00

TUBA1A / TUBA1B / TUBA1C / TUBA3C / TUBA8 / TUBA4A Antibody

20-abx329824
  • EUR 314.00
  • EUR 244.00
  • 0
  • 1
  • Shipped within 5-10 working days.

TUBA8 sgRNA CRISPR Lentivector (Human) (Target 1)

K2557902 1.0 ug DNA
EUR 154.00

TUBA8 sgRNA CRISPR Lentivector (Human) (Target 2)

K2557903 1.0 ug DNA
EUR 154.00

TUBA8 sgRNA CRISPR Lentivector (Human) (Target 3)

K2557904 1.0 ug DNA
EUR 154.00

Tuba8 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3429402 1.0 ug DNA
EUR 154.00

Tuba8 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3429403 1.0 ug DNA
EUR 154.00

Tuba8 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3429404 1.0 ug DNA
EUR 154.00

TUBA1A/TUBA1B/TUBA1C/TUBA3C/TUBA8/TUBA4A Antibody

1-CSB-PA193238
  • EUR 222.00
  • EUR 195.00
  • 0
  • 1
  • Form: Liquid
  • Buffer: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Description: A polyclonal antibody against TUBA1A/TUBA1B/TUBA1C/TUBA3C/TUBA8/TUBA4A. Recognizes TUBA1A/TUBA1B/TUBA1C/TUBA3C/TUBA8/TUBA4A from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1:500-2000, ELISA:1:10000-20000

Tuba8 sgRNA CRISPR Lentivector (Rat) (Target 1)

K6710902 1.0 ug DNA
EUR 154.00

Tuba8 sgRNA CRISPR Lentivector (Rat) (Target 2)

K6710903 1.0 ug DNA
EUR 154.00

Tuba8 sgRNA CRISPR Lentivector (Rat) (Target 3)

K6710904 1.0 ug DNA
EUR 154.00

Human Tubulin Alpha 8(TUBa8)ELISA Kit

QY-E01102 96T
EUR 361.00

TUBA8 Protein Vector (Rat) (pPB-C-His)

PV313694 500 ng
EUR 603.00

TUBA8 Protein Vector (Rat) (pPB-N-His)

PV313695 500 ng
EUR 603.00

TUBA8 Protein Vector (Rat) (pPM-C-HA)

PV313696 500 ng
EUR 603.00

TUBA8 Protein Vector (Rat) (pPM-C-His)

PV313697 500 ng
EUR 603.00

TUBA8 Protein Vector (Human) (pPB-C-His)

PV141270 500 ng
EUR 552.00

TUBA8 Protein Vector (Human) (pPB-N-His)

PV141271 500 ng
EUR 552.00

TUBA8 Protein Vector (Human) (pPM-C-HA)

PV141272 500 ng
EUR 552.00

TUBA8 Protein Vector (Human) (pPM-C-His)

PV141273 500 ng
EUR 552.00

TUBA8 Protein Vector (Mouse) (pPB-C-His)

PV242974 500 ng
EUR 603.00

TUBA8 Protein Vector (Mouse) (pPB-N-His)

PV242975 500 ng
EUR 603.00

TUBA8 Protein Vector (Mouse) (pPM-C-HA)

PV242976 500 ng
EUR 603.00

TUBA8 Protein Vector (Mouse) (pPM-C-His)

PV242977 500 ng
EUR 603.00

Tuba8 3'UTR GFP Stable Cell Line

TU171355 1.0 ml Ask for price

TUBA8 3'UTR GFP Stable Cell Line

TU077512 1.0 ml
EUR 1394.00

Tuba8 3'UTR Luciferase Stable Cell Line

TU121355 1.0 ml Ask for price

TUBA8 3'UTR Luciferase Stable Cell Line

TU027512 1.0 ml
EUR 1394.00

Tuba8 3'UTR Luciferase Stable Cell Line

TU222652 1.0 ml Ask for price

Tuba8 3'UTR GFP Stable Cell Line

TU272652 1.0 ml Ask for price

Human Tubulin alpha- 8 chain, TUBA8 ELISA KIT

ELI-53133h 96 Tests
EUR 824.00

Bovine Tubulin alpha- 8 chain, TUBA8 ELISA KIT

ELI-29737b 96 Tests
EUR 928.00

Mouse Tubulin alpha- 8 chain, Tuba8 ELISA KIT

ELI-41926m 96 Tests
EUR 865.00

TUBA8 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV680851 1.0 ug DNA
EUR 682.00

TUBA8 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV680855 1.0 ug DNA
EUR 682.00

TUBA8 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV680856 1.0 ug DNA
EUR 682.00

TUBA8 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K2557905 3 x 1.0 ug
EUR 376.00

Tuba8 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K3429405 3 x 1.0 ug
EUR 376.00

Tuba8 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K6710905 3 x 1.0 ug
EUR 376.00

TUBA8 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K2557906 1.0 ug DNA
EUR 167.00

TUBA8 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K2557907 1.0 ug DNA
EUR 167.00

TUBA8 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K2557908 1.0 ug DNA
EUR 167.00

Tuba8 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K3429406 1.0 ug DNA
EUR 167.00

Tuba8 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K3429407 1.0 ug DNA
EUR 167.00

Tuba8 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K3429408 1.0 ug DNA
EUR 167.00

TUBA8 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV680852 1.0 ug DNA
EUR 682.00

TUBA8 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV680853 1.0 ug DNA
EUR 740.00

TUBA8 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV680854 1.0 ug DNA
EUR 740.00

Tuba8 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)

K6710906 1.0 ug DNA
EUR 167.00

Tuba8 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)

K6710907 1.0 ug DNA
EUR 167.00

Tuba8 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)

K6710908 1.0 ug DNA
EUR 167.00

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