Clinical application and evaluation of metagenomic next-generation sequencing in suspected adult central nervous system infection.

accurate etiologic diagnosis is essential for infection of the central nervous system (CNS infections). The diagnostic value of metagenomic sequencing generation (mNGS), a powerful platform that appears, remains to be studied in the CNS infections.

We conduct a prospective cohort study comparing single-center for mNGS with conventional methods including culture, smear and others 248 suspected CNS infectious patients were enrolled and clinical data were recorded.


RESULTS
mNGS reported a sensitivity of 90.00% (9/10) in patients with culture-positive without empirical treatment and 66.67% (6/9) in patients treated empirically. Detected addition of 48 bacteria and fungi in culture-negative patients, mNGS provide higher detection rate compared with culture in patients with (34.45% vs. 7.56%, McNemar test, p <0.0083) or without empirical therapy ( 50.00% vs 25.00%, McNemar test, p> 0.0083).

Compared with the conventional method, the positive percent agreement and negative percent agreement was 75.00% and 69.11% separately. mNGS detection rate was significantly higher in patients with cerebrospinal fluid (CSF) WBC> 300 * 106 / L, CSF protein> 500 mg / L or glucose ratio ≤ 0.3. mNGS sequencing reads correlated with CSF WBC, the ratio of glucose and the development of clinical disease.


mNGS showed satisfactory diagnostic performance in CNS infections and has a superior detection rate overall culture. mNGS probably held diagnostic benefit, especially in patients treated empirically. CSF laboratory results are statistically relevant mNGS detection rate, and mNGS can dynamically monitor the progression of the disease.

accurate etiologic diagnosis is essential for infection of the central nervous system (CNS infections). The diagnostic value of metagenomic sequencing generation (mNGS), a powerful platform that appears, remains to be studied in the CNS infections.We conduct a prospective cohort study comparing single-center for mNGS with conventional methods including culture, smear and others 248 suspected CNS infectious patients were enrolled and clinical data were recorded. RESULTS mNGS reported a sensitivity of 90.00% (9/10) in patients with culture-positive without empirical treatment and 66.67% (6/9) in patients treated empirically. Detected addition of 48 bacteria and fungi in culture-negative patients, mNGS provide higher detection rate compared with culture in patients with (34.45% vs. 7.56%, McNemar test, p 0.0083). Compared with the conventional method, the positive percent agreement and negative percent agreement was 75.00% and 69.11% separately. mNGS detection rate was significantly higher in patients with cerebrospinal fluid (CSF) WBC> 300 * 106 / L, CSF protein> 500 mg / L or glucose ratio ≤ 0.3. mNGS sequencing reads correlated with CSF WBC, the ratio of glucose and the development of clinical disease. mNGS showed satisfactory diagnostic performance in CNS infections and has a superior detection rate overall culture. mNGS probably held diagnostic benefit, especially in patients treated empirically. CSF laboratory results are statistically relevant mNGS detection rate, and mNGS can dynamically monitor the progression of the disease.
accurate etiologic diagnosis is essential for infection of the central nervous system (CNS infections). The diagnostic value of metagenomic sequencing generation (mNGS), a powerful platform that appears, remains to be studied in the CNS infections.We conduct a prospective cohort study comparing single-center for mNGS with conventional methods including culture, smear and others 248 suspected CNS infectious patients were enrolled and clinical data were recorded. RESULTS mNGS reported a sensitivity of 90.00% (9/10) in patients with culture-positive without empirical treatment and 66.67% (6/9) in patients treated empirically. Detected addition of 48 bacteria and fungi in culture-negative patients, mNGS provide higher detection rate compared with culture in patients with (34.45% vs. 7.56%, McNemar test, p <0.0083) or without empirical therapy ( 50.00% vs 25.00%, McNemar test, p> 0.0083). Compared with the conventional method, the positive percent agreement and negative percent agreement was 75.00% and 69.11% separately. mNGS detection rate was significantly higher in patients with cerebrospinal fluid (CSF) WBC> 300 * 106 / L, CSF protein> 500 mg / L or glucose ratio ≤ 0.3. mNGS sequencing reads correlated with CSF WBC, the ratio of glucose and the development of clinical disease. mNGS showed satisfactory diagnostic performance in CNS infections and has a superior detection rate overall culture. mNGS probably held diagnostic benefit, especially in patients treated empirically. CSF laboratory results are statistically relevant mNGS detection rate, and mNGS can dynamically monitor the progression of the disease.

TRMT112 Rabbit pAb

A14310-100ul 100 ul
EUR 369.6

TRMT112 Rabbit pAb

A14310-200ul 200 ul
EUR 550.8

TRMT112 Rabbit pAb

A14310-20ul 20 ul
EUR 219.6

TRMT112 Rabbit pAb

A14310-50ul 50 ul
EUR 267.6

TRMT112 Conjugated Antibody

C47640 100ul
EUR 476.4

Anti-TRMT112 antibody

STJ116522 100 µl
EUR 332.4

TRMT112 Polyclonal Antibody

A55451-020 20 ul
EUR 117.7

TRMT112 Polyclonal Antibody

A55451-050 50 ul
EUR 302.5

TRMT112 Polyclonal Antibody

A55451-100 100 ul
EUR 423.5

TRMT112 Polyclonal Antibody

A55451
  • EUR 684.66
  • EUR 117.70
  • EUR 302.50
  • EUR 423.50
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

TRMT112 Polyclonal Antibody

A71570
  • EUR 302.50
  • EUR 423.50
  • 50 ul
  • 100 ul

TRMT112 Polyclonal Antibody

A71570-050 50 ul
EUR 302.5

TRMT112 Polyclonal Antibody

A71570-100 100 ul
EUR 423.5

TRMT112 Antibody, HRP conjugated

1-CSB-PA883416LB01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against TRMT112. Recognizes TRMT112 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

TRMT112 Antibody, FITC conjugated

1-CSB-PA883416LC01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against TRMT112. Recognizes TRMT112 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

TRMT112 Antibody, Biotin conjugated

1-CSB-PA883416LD01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against TRMT112. Recognizes TRMT112 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Human TRMT112 shRNA Plasmid

20-abx959798
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Mouse TRMT112 shRNA Plasmid

20-abx976259
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

TRMT112 Recombinant Protein (Rat)

RP234806 100 ug Ask for price

TRMT112 Recombinant Protein (Mouse)

RP181355 100 ug Ask for price

TRMT112 Recombinant Protein (Mouse)

RP181358 100 ug Ask for price

TRMT112 Recombinant Protein (Human)

RP103622 100 ug Ask for price

Trmt112 ORF Vector (Rat) (pORF)

ORF078270 1.0 ug DNA
EUR 607.2

Trmt112 ORF Vector (Mouse) (pORF)

ORF060453 1.0 ug DNA
EUR 607.2

Trmt112 ORF Vector (Mouse) (pORF)

ORF060454 1.0 ug DNA
EUR 607.2

TRMT112 ORF Vector (Human) (pORF)

ORF034542 1.0 ug DNA Ask for price

TRMT112 Polyclonal Antibody, HRP Conjugated

A55452-050 50 ul
EUR 302.5

TRMT112 Polyclonal Antibody, HRP Conjugated

A55452-100 100 ul
EUR 423.5

TRMT112 Polyclonal Antibody, FITC Conjugated

A55453-050 50 ul
EUR 302.5

TRMT112 Polyclonal Antibody, FITC Conjugated

A55453-100 100 ul
EUR 423.5

TRMT112 Polyclonal Antibody, Biotin Conjugated

A55454-050 50 ul
EUR 302.5

TRMT112 Polyclonal Antibody, Biotin Conjugated

A55454-100 100 ul
EUR 423.5

TRMT112 Polyclonal Antibody, HRP Conjugated

A55452
  • EUR 684.66
  • EUR 302.50
  • EUR 423.50
  • 100 µg
  • 50 ul
  • 100 ul

TRMT112 Polyclonal Antibody, FITC Conjugated

A55453
  • EUR 684.66
  • EUR 302.50
  • EUR 423.50
  • 100 µg
  • 50 ul
  • 100 ul

TRMT112 Polyclonal Antibody, Biotin Conjugated

A55454
  • EUR 684.66
  • EUR 302.50
  • EUR 423.50
  • 100 µg
  • 50 ul
  • 100 ul

tRNA Methyltransferase 112 Homolog (TRMT112) Antibody

20-abx110297
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Human tRNA methyltransferase 112 homolog (TRMT112)

1-CSB-EP883416HU
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human tRNA methyltransferase 112 homolog(TRMT112) expressed in E.coli

Trmt112 sgRNA CRISPR Lentivector set (Rat)

K6675801 3 x 1.0 ug
EUR 406.8

Trmt112 sgRNA CRISPR Lentivector set (Mouse)

K3430401 3 x 1.0 ug
EUR 406.8

TRMT112 sgRNA CRISPR Lentivector set (Human)

K2814901 3 x 1.0 ug
EUR 406.8

Trmt112 sgRNA CRISPR Lentivector (Rat) (Target 1)

K6675802 1.0 ug DNA
EUR 184.8

Trmt112 sgRNA CRISPR Lentivector (Rat) (Target 2)

K6675803 1.0 ug DNA
EUR 184.8

Trmt112 sgRNA CRISPR Lentivector (Rat) (Target 3)

K6675804 1.0 ug DNA
EUR 184.8

Trmt112 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3430402 1.0 ug DNA
EUR 184.8

Trmt112 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3430403 1.0 ug DNA
EUR 184.8

Trmt112 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3430404 1.0 ug DNA
EUR 184.8

TRMT112 sgRNA CRISPR Lentivector (Human) (Target 1)

K2814902 1.0 ug DNA
EUR 184.8

TRMT112 sgRNA CRISPR Lentivector (Human) (Target 2)

K2814903 1.0 ug DNA
EUR 184.8

TRMT112 sgRNA CRISPR Lentivector (Human) (Target 3)

K2814904 1.0 ug DNA
EUR 184.8

TRMT112 Protein Vector (Human) (pPB-C-His)

PV138166 500 ng Ask for price

TRMT112 Protein Vector (Human) (pPB-N-His)

PV138167 500 ng Ask for price

TRMT112 Protein Vector (Human) (pPM-C-HA)

PV138168 500 ng Ask for price

TRMT112 Protein Vector (Human) (pPM-C-His)

PV138169 500 ng Ask for price

TRMT112 3'UTR GFP Stable Cell Line

TU076628 1.0 ml
EUR 2799.6

Trmt112 3'UTR GFP Stable Cell Line

TU272483 1.0 ml Ask for price

Trmt112 3'UTR Luciferase Stable Cell Line

TU222483 1.0 ml Ask for price

Trmt112 3'UTR GFP Stable Cell Line

TU171152 1.0 ml Ask for price

TRMT112 Protein Vector (Rat) (pPB-C-His)

PV313078 500 ng
EUR 723.6

TRMT112 Protein Vector (Rat) (pPB-N-His)

PV313079 500 ng
EUR 723.6

TRMT112 Protein Vector (Rat) (pPM-C-HA)

PV313080 500 ng
EUR 723.6

TRMT112 Protein Vector (Rat) (pPM-C-His)

PV313081 500 ng
EUR 723.6

TRMT112 Protein Vector (Mouse) (pPB-C-His)

PV241810 500 ng
EUR 723.6

TRMT112 Protein Vector (Mouse) (pPB-N-His)

PV241811 500 ng
EUR 723.6

TRMT112 Protein Vector (Mouse) (pPM-C-HA)

PV241812 500 ng
EUR 723.6

TRMT112 Protein Vector (Mouse) (pPM-C-His)

PV241813 500 ng
EUR 723.6

TRMT112 Protein Vector (Mouse) (pPB-C-His)

PV241814 500 ng
EUR 723.6

TRMT112 Protein Vector (Mouse) (pPB-N-His)

PV241815 500 ng
EUR 723.6

TRMT112 Protein Vector (Mouse) (pPM-C-HA)

PV241816 500 ng
EUR 723.6

TRMT112 Protein Vector (Mouse) (pPM-C-His)

PV241817 500 ng
EUR 723.6

Trmt112 3'UTR Luciferase Stable Cell Line

TU121152 1.0 ml Ask for price

TRMT112 3'UTR Luciferase Stable Cell Line

TU026628 1.0 ml
EUR 2799.6

Human tRNA methyltransferase 112 homolog, TRMT112 ELISA KIT

ELI-23070h 96 Tests
EUR 988.8

Bovine tRNA methyltransferase 112 homolog, TRMT112 ELISA KIT

ELI-45595b 96 Tests
EUR 1113.6

TRMT112 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV644191 1.0 ug DNA
EUR 616.8

TRMT112 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV644195 1.0 ug DNA
EUR 616.8

TRMT112 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV644196 1.0 ug DNA
EUR 616.8

Mouse tRNA methyltransferase 112 homolog, Trmt112 ELISA KIT

ELI-16498m 96 Tests
EUR 1038

Recombinant Human TRMT112 Protein, His-SUMO, E.coli-100ug

QP8188-ec-100ug 100ug
EUR 489.6

Recombinant Human TRMT112 Protein, His-SUMO, E.coli-10ug

QP8188-ec-10ug 10ug
EUR 240

Recombinant Human TRMT112 Protein, His-SUMO, E.coli-1mg

QP8188-ec-1mg 1mg
EUR 1958.4

Recombinant Human TRMT112 Protein, His-SUMO, E.coli-200ug

QP8188-ec-200ug 200ug
EUR 760.8

Recombinant Human TRMT112 Protein, His-SUMO, E.coli-500ug

QP8188-ec-500ug 500ug
EUR 1272

Recombinant Human TRMT112 Protein, His-SUMO, E.coli-50ug

QP8188-ec-50ug 50ug
EUR 315.6

Trmt112 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K6675805 3 x 1.0 ug
EUR 451.2

Trmt112 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K3430405 3 x 1.0 ug
EUR 451.2

TRMT112 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K2814905 3 x 1.0 ug
EUR 451.2

Trmt112 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)

K6675806 1.0 ug DNA
EUR 200.4

Trmt112 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)

K6675807 1.0 ug DNA
EUR 200.4

Trmt112 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)

K6675808 1.0 ug DNA
EUR 200.4

TRMT112 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV644192 1.0 ug DNA
EUR 616.8

TRMT112 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV644193 1.0 ug DNA
EUR 686.4

TRMT112 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV644194 1.0 ug DNA
EUR 686.4

Trmt112 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K3430406 1.0 ug DNA
EUR 200.4

Trmt112 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K3430407 1.0 ug DNA
EUR 200.4

Trmt112 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K3430408 1.0 ug DNA
EUR 200.4

TRMT112 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K2814906 1.0 ug DNA
EUR 200.4

TRMT112 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K2814907 1.0 ug DNA
EUR 200.4

TRMT112 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K2814908 1.0 ug DNA
EUR 200.4

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