Clinical application and evaluation of metagenomic next-generation sequencing in suspected adult central nervous system infection.

accurate etiologic diagnosis is essential for infection of the central nervous system (CNS infections). The diagnostic value of metagenomic sequencing generation (mNGS), a powerful platform that appears, remains to be studied in the CNS infections.

We conduct a prospective cohort study comparing single-center for mNGS with conventional methods including culture, smear and others 248 suspected CNS infectious patients were enrolled and clinical data were recorded.


RESULTS
mNGS reported a sensitivity of 90.00% (9/10) in patients with culture-positive without empirical treatment and 66.67% (6/9) in patients treated empirically. Detected addition of 48 bacteria and fungi in culture-negative patients, mNGS provide higher detection rate compared with culture in patients with (34.45% vs. 7.56%, McNemar test, p <0.0083) or without empirical therapy ( 50.00% vs 25.00%, McNemar test, p> 0.0083).

Compared with the conventional method, the positive percent agreement and negative percent agreement was 75.00% and 69.11% separately. mNGS detection rate was significantly higher in patients with cerebrospinal fluid (CSF) WBC> 300 * 106 / L, CSF protein> 500 mg / L or glucose ratio ≤ 0.3. mNGS sequencing reads correlated with CSF WBC, the ratio of glucose and the development of clinical disease.


mNGS showed satisfactory diagnostic performance in CNS infections and has a superior detection rate overall culture. mNGS probably held diagnostic benefit, especially in patients treated empirically. CSF laboratory results are statistically relevant mNGS detection rate, and mNGS can dynamically monitor the progression of the disease.

accurate etiologic diagnosis is essential for infection of the central nervous system (CNS infections). The diagnostic value of metagenomic sequencing generation (mNGS), a powerful platform that appears, remains to be studied in the CNS infections.We conduct a prospective cohort study comparing single-center for mNGS with conventional methods including culture, smear and others 248 suspected CNS infectious patients were enrolled and clinical data were recorded. RESULTS mNGS reported a sensitivity of 90.00% (9/10) in patients with culture-positive without empirical treatment and 66.67% (6/9) in patients treated empirically. Detected addition of 48 bacteria and fungi in culture-negative patients, mNGS provide higher detection rate compared with culture in patients with (34.45% vs. 7.56%, McNemar test, p 0.0083). Compared with the conventional method, the positive percent agreement and negative percent agreement was 75.00% and 69.11% separately. mNGS detection rate was significantly higher in patients with cerebrospinal fluid (CSF) WBC> 300 * 106 / L, CSF protein> 500 mg / L or glucose ratio ≤ 0.3. mNGS sequencing reads correlated with CSF WBC, the ratio of glucose and the development of clinical disease. mNGS showed satisfactory diagnostic performance in CNS infections and has a superior detection rate overall culture. mNGS probably held diagnostic benefit, especially in patients treated empirically. CSF laboratory results are statistically relevant mNGS detection rate, and mNGS can dynamically monitor the progression of the disease.
accurate etiologic diagnosis is essential for infection of the central nervous system (CNS infections). The diagnostic value of metagenomic sequencing generation (mNGS), a powerful platform that appears, remains to be studied in the CNS infections.We conduct a prospective cohort study comparing single-center for mNGS with conventional methods including culture, smear and others 248 suspected CNS infectious patients were enrolled and clinical data were recorded. RESULTS mNGS reported a sensitivity of 90.00% (9/10) in patients with culture-positive without empirical treatment and 66.67% (6/9) in patients treated empirically. Detected addition of 48 bacteria and fungi in culture-negative patients, mNGS provide higher detection rate compared with culture in patients with (34.45% vs. 7.56%, McNemar test, p <0.0083) or without empirical therapy ( 50.00% vs 25.00%, McNemar test, p> 0.0083). Compared with the conventional method, the positive percent agreement and negative percent agreement was 75.00% and 69.11% separately. mNGS detection rate was significantly higher in patients with cerebrospinal fluid (CSF) WBC> 300 * 106 / L, CSF protein> 500 mg / L or glucose ratio ≤ 0.3. mNGS sequencing reads correlated with CSF WBC, the ratio of glucose and the development of clinical disease. mNGS showed satisfactory diagnostic performance in CNS infections and has a superior detection rate overall culture. mNGS probably held diagnostic benefit, especially in patients treated empirically. CSF laboratory results are statistically relevant mNGS detection rate, and mNGS can dynamically monitor the progression of the disease.

TRMT112 Antibody

47640-100ul 100ul
EUR 252.00

TRMT112 Antibody

1-CSB-PA883416LA01HU
  • EUR 317.00
  • EUR 335.00
  • 0
  • 1
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against TRMT112. Recognizes TRMT112 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

TRMT112 Conjugated Antibody

C47640 100ul
EUR 397.00

TRMT112 Polyclonal Antibody

A55451 100 µg
EUR 570.55
Description: fast delivery possible

TRMT112 Rabbit pAb

A14310-100ul 100 ul
EUR 308.00

TRMT112 Rabbit pAb

A14310-200ul 200 ul
EUR 459.00

TRMT112 Rabbit pAb

A14310-20ul 20 ul
EUR 183.00

TRMT112 Rabbit pAb

A14310-50ul 50 ul
EUR 223.00

Anti-TRMT112 antibody

STJ116522 100 µl
EUR 277.00

Mouse TRMT112 shRNA Plasmid

20-abx976259
  • EUR 801.00
  • EUR 1121.00
  • 0
  • 1
  • Shipped within 15-20 working days.

Human TRMT112 shRNA Plasmid

20-abx959798
  • EUR 801.00
  • EUR 1121.00
  • 0
  • 1
  • Shipped within 15-20 working days.

TRMT112 Antibody, HRP conjugated

1-CSB-PA883416LB01HU
  • EUR 317.00
  • EUR 335.00
  • 0
  • 1
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against TRMT112. Recognizes TRMT112 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

TRMT112 Antibody, FITC conjugated

1-CSB-PA883416LC01HU
  • EUR 317.00
  • EUR 335.00
  • 0
  • 1
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against TRMT112. Recognizes TRMT112 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

TRMT112 Antibody, Biotin conjugated

1-CSB-PA883416LD01HU
  • EUR 317.00
  • EUR 335.00
  • 0
  • 1
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against TRMT112. Recognizes TRMT112 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

TRMT112 Recombinant Protein (Human)

RP103622 100 ug Ask for price

TRMT112 Recombinant Protein (Rat)

RP234806 100 ug Ask for price

TRMT112 Recombinant Protein (Mouse)

RP181355 100 ug Ask for price

TRMT112 Recombinant Protein (Mouse)

RP181358 100 ug Ask for price

TRMT112 Polyclonal Antibody, HRP Conjugated

A55452 100 µg
EUR 570.55
Description: reagents widely cited

TRMT112 Polyclonal Antibody, FITC Conjugated

A55453 100 µg
EUR 570.55
Description: Ask the seller for details

TRMT112 Polyclonal Antibody, Biotin Conjugated

A55454 100 µg
EUR 570.55
Description: The best epigenetics products

TRMT112 ORF Vector (Human) (pORF)

ORF034542 1.0 ug DNA Ask for price

Trmt112 ORF Vector (Rat) (pORF)

ORF078270 1.0 ug DNA
EUR 506.00

Trmt112 ORF Vector (Mouse) (pORF)

ORF060453 1.0 ug DNA
EUR 506.00

Trmt112 ORF Vector (Mouse) (pORF)

ORF060454 1.0 ug DNA
EUR 506.00

tRNA Methyltransferase 112 Homolog (TRMT112) Antibody

20-abx110297
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 0
  • 1
  • 2
  • 3
  • 4
  • Shipped within 5-10 working days.

TRMT112 sgRNA CRISPR Lentivector set (Human)

K2814901 3 x 1.0 ug
EUR 339.00

Trmt112 sgRNA CRISPR Lentivector set (Mouse)

K3430401 3 x 1.0 ug
EUR 339.00

Human tRNA methyltransferase 112 homolog (TRMT112)

1-CSB-EP883416HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 0
  • 1
  • 2
  • 3
  • 4
  • 5
  • MW: 30.2 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human tRNA methyltransferase 112 homolog(TRMT112) expressed in E.coli

Trmt112 sgRNA CRISPR Lentivector set (Rat)

K6675801 3 x 1.0 ug
EUR 339.00

TRMT112 sgRNA CRISPR Lentivector (Human) (Target 1)

K2814902 1.0 ug DNA
EUR 154.00

TRMT112 sgRNA CRISPR Lentivector (Human) (Target 2)

K2814903 1.0 ug DNA
EUR 154.00

TRMT112 sgRNA CRISPR Lentivector (Human) (Target 3)

K2814904 1.0 ug DNA
EUR 154.00

Trmt112 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3430402 1.0 ug DNA
EUR 154.00

Trmt112 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3430403 1.0 ug DNA
EUR 154.00

Trmt112 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3430404 1.0 ug DNA
EUR 154.00

Trmt112 sgRNA CRISPR Lentivector (Rat) (Target 1)

K6675802 1.0 ug DNA
EUR 154.00

Trmt112 sgRNA CRISPR Lentivector (Rat) (Target 2)

K6675803 1.0 ug DNA
EUR 154.00

Trmt112 sgRNA CRISPR Lentivector (Rat) (Target 3)

K6675804 1.0 ug DNA
EUR 154.00

TRMT112 Protein Vector (Rat) (pPB-C-His)

PV313078 500 ng
EUR 603.00

TRMT112 Protein Vector (Rat) (pPB-N-His)

PV313079 500 ng
EUR 603.00

TRMT112 Protein Vector (Rat) (pPM-C-HA)

PV313080 500 ng
EUR 603.00

TRMT112 Protein Vector (Rat) (pPM-C-His)

PV313081 500 ng
EUR 603.00

TRMT112 Protein Vector (Human) (pPB-C-His)

PV138166 500 ng Ask for price

TRMT112 Protein Vector (Human) (pPB-N-His)

PV138167 500 ng Ask for price

TRMT112 Protein Vector (Human) (pPM-C-HA)

PV138168 500 ng Ask for price

TRMT112 Protein Vector (Human) (pPM-C-His)

PV138169 500 ng Ask for price

TRMT112 Protein Vector (Mouse) (pPB-C-His)

PV241810 500 ng
EUR 603.00

TRMT112 Protein Vector (Mouse) (pPB-N-His)

PV241811 500 ng
EUR 603.00

TRMT112 Protein Vector (Mouse) (pPM-C-HA)

PV241812 500 ng
EUR 603.00

TRMT112 Protein Vector (Mouse) (pPM-C-His)

PV241813 500 ng
EUR 603.00

TRMT112 Protein Vector (Mouse) (pPB-C-His)

PV241814 500 ng
EUR 603.00

TRMT112 Protein Vector (Mouse) (pPB-N-His)

PV241815 500 ng
EUR 603.00

TRMT112 Protein Vector (Mouse) (pPM-C-HA)

PV241816 500 ng
EUR 603.00

TRMT112 Protein Vector (Mouse) (pPM-C-His)

PV241817 500 ng
EUR 603.00

Trmt112 3'UTR GFP Stable Cell Line

TU171152 1.0 ml Ask for price

TRMT112 3'UTR GFP Stable Cell Line

TU076628 1.0 ml
EUR 2333.00

Trmt112 3'UTR Luciferase Stable Cell Line

TU121152 1.0 ml Ask for price

TRMT112 3'UTR Luciferase Stable Cell Line

TU026628 1.0 ml
EUR 2333.00

Trmt112 3'UTR Luciferase Stable Cell Line

TU222483 1.0 ml Ask for price

Trmt112 3'UTR GFP Stable Cell Line

TU272483 1.0 ml Ask for price

Human tRNA methyltransferase 112 homolog, TRMT112 ELISA KIT

ELI-23070h 96 Tests
EUR 824.00

Mouse tRNA methyltransferase 112 homolog, Trmt112 ELISA KIT

ELI-16498m 96 Tests
EUR 865.00

Bovine tRNA methyltransferase 112 homolog, TRMT112 ELISA KIT

ELI-45595b 96 Tests
EUR 928.00

TRMT112 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV644191 1.0 ug DNA
EUR 514.00

TRMT112 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV644195 1.0 ug DNA
EUR 514.00

TRMT112 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV644196 1.0 ug DNA
EUR 514.00

Recombinant Human TRMT112 Protein, His-SUMO, E.coli-100ug

QP8188-ec-100ug 100ug
EUR 408.00

Recombinant Human TRMT112 Protein, His-SUMO, E.coli-10ug

QP8188-ec-10ug 10ug
EUR 200.00

Recombinant Human TRMT112 Protein, His-SUMO, E.coli-1mg

QP8188-ec-1mg 1mg
EUR 1632.00

Recombinant Human TRMT112 Protein, His-SUMO, E.coli-200ug

QP8188-ec-200ug 200ug
EUR 634.00

Recombinant Human TRMT112 Protein, His-SUMO, E.coli-500ug

QP8188-ec-500ug 500ug
EUR 1060.00

Recombinant Human TRMT112 Protein, His-SUMO, E.coli-50ug

QP8188-ec-50ug 50ug
EUR 263.00

TRMT112 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K2814905 3 x 1.0 ug
EUR 376.00

Trmt112 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K3430405 3 x 1.0 ug
EUR 376.00

Trmt112 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K6675805 3 x 1.0 ug
EUR 376.00

TRMT112 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K2814906 1.0 ug DNA
EUR 167.00

TRMT112 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K2814907 1.0 ug DNA
EUR 167.00

TRMT112 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K2814908 1.0 ug DNA
EUR 167.00

Trmt112 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K3430406 1.0 ug DNA
EUR 167.00

Trmt112 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K3430407 1.0 ug DNA
EUR 167.00

Trmt112 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K3430408 1.0 ug DNA
EUR 167.00

TRMT112 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV644192 1.0 ug DNA
EUR 514.00

TRMT112 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV644193 1.0 ug DNA
EUR 572.00

TRMT112 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV644194 1.0 ug DNA
EUR 572.00

Trmt112 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)

K6675806 1.0 ug DNA
EUR 167.00

Trmt112 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)

K6675807 1.0 ug DNA
EUR 167.00

Trmt112 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)

K6675808 1.0 ug DNA
EUR 167.00

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